t/data/primer3_output.txt

   1 PRIMER_SEQUENCE_ID=XXU25548
   2 SEQUENCE=CTGACTCTTATACACAAGTAGCGTCCTGGACGGAACCTTTCCCGTTTTGCCCTGTTCTGGAAAACCGGGCTGCTCAGGGCGATATTACTGCACCCGGCGGTGCTCGCCGTTTAACGGGTGATCAGACTGCCGCTCTGCGTGATTCTCTTAGCGATAAACCTGCAAAAAATATTATTTTGCTGATTGGCGATGGGATGGGGGACTCGGAAATTACTGCCGCACGTAATTATGCCGAAGGTGCGGGCGGCTTTTTTAAAGGTATAGATGCCTTACCGCTTACCGGGCAATACACTCACTATGCGCTGAATAAAAAAACCGGCAAACCGGACTACGTCACCGACTCGGCTGCATCAGCAACCGCCTGGTCAACCGGTGTCAAAACCTATAACGGCGCGCTGGGCGTCGATATTCACGAAAAAGATCACCCAACGATTCTGGAAATGGCAAAAGCCGCAGGTCTGGCGACCGGTAACGTTTCTACCGCAGAGTTGCAGGATGCCACGCCCGCTGCGCTGGTGGCACATGTGACCTCGCGCAAATGCTACGGTCCGAGCGCGACCAGTGAAAAATGTCCGGGTAACGCTCTGGAAAAAGGCGGAAAAGGATCGATTACCGAACAGCTGCTTAACGCTCGTGCCGACGTTACGCTTGGCGGCGGCGCAAAAACCTTTGCTGAAACGGCAACCGCTGGTGAATGGCAGGGAAAAACGCTGCGTGAACAGGCACAGGCGCGTGGTTATCAGTTGGTGAGCGATGCTGCCTCACTGAATTCGGTGACGGAAGCGAATCAGCAAAAACCCCTGCTTGGCCTGTTTGCTGACGGCAATATGCCAGTGCGCTGGCTAGGACCGAAAGCAACGTACCATGGCAATATCGATAAGCCCGCAGTCACCTGTACGCCAAATCCGCAACGTAATGACAGTGTACCAACCCTGGCGCAGATGACCGACAAAGCCATTGAATTGTTGAGTAAAAATGAGAAAGGCTTTTTCCTGCAAGTTGAAGGTGCGTCAATCGATAAACAGGATCATGCTGCGAATCCTTGTGGGCAAATTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCGGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTAACCACCGTCGGCGCTGCCGAGAGAACTTTAACCCCACAACAACAGCGTATGACCTCCTGTAATCAGCAGGCGACGGCGCAGGCGTTGAAAGGGGATGCTCGTAAGACCTACATGAGTGATTGCCTGAAGAACAGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAATGATGAAAACTTTTTCAAAAAAGCGGCGGCGCACGGGGAGGAACCTCCTTTAACTCCTCAAAACGAACATCAGCGGTCCGGGCTGCGCTTCGCCCGTCGCGTCAGACTACCCCGTGCGGTTGGCCTGGCTGGCATGTTCTTACCGATTGCTTCAACGCTGGTTTCACACCCGCCGCCGGGCTGGTGGTGGCTGGTGTTGGTCGGCTGGGCGTTCGTCTGGCCGCATTTAGCCTGGCAGATAGCGAGCAGGGCCGTCGATCCGCTTAGCCGGGAAATTTACAACTTAAAAACCGATGCAGTATTAGCGGGAATGTGGGTAGGCGTAATGGGCGTAAACGTGCTGCCTTCCACCGCGATGTTGATGATTATGTGTCTGAATTTGATGGGGGCAGGCGGCCCCCGTCTGTTTGTCGCGGGTCTGGTGTTGATGGTGGTTTCCTGCCTTGTCACCCTCGAGGCCACCACATTCCGCACCGTAGGATTACTGCATCAGGAGTGGTGGATGCGCCCGGATGACCCTGCCGATGCGGATGAAAAGGAGAGTGGCAAATGGCTGGCAGCGGCCGCAACTAGCCGGTTACGCATGGGCAGCATGATGAGCAACGTGATTGCGGTCTGTGACCGCGAAGCCGATATTCATGCTTATCTGCAGGACAGGCTGGCGCATAACGAGCGCTTCGTGGTGCGCTCCAAGCACCCACGCAAGGACGTAGAGTCTGGGTTGTATCTGATCGACCATCTGAAGAACCAACCGGAGTTGGGTGGCTATCAGATCAGCATTCCGCAAAAGGGCGTGGTGGATAAACGCGGTAAACGTAAAAATCGACCAGCCCGCAAGGCGAGCTTGAGCCTGCGCAGTGGGCGCATCACGCTAAAACAGGGGAATATCACGCTCAACGCGGTGCTGGCCGAGGAGATTAACCCGCCCAAGGGTGAGACCCCGTTGAAATGGTTGTTGCTGACCGGCGAACCGGTCGAGTCGCTAGCCCAAGCCTTGCGCGTCATCGACATTTATACCCATCGCTGGCGGATCGAGGAGTTCCATAAGGCATGGAAAACCGGAGCAGGAGCCGAGAGGCAACGCATGGAGGAGCCGGATAATCTGGAGCGGATGGTCTCGATCCTCTCGTTTGTTGCGGTCAGGCTGTTACAGCTCAGAGAAAGCTTCACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCGGGCTCGATCCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTCGCGGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCGCATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGATGGCCGCTTTGGTCGACCCGGACGGGACGCTCCTGCGCCTGATACAGAACGAATTGCTTGCAGGCATCTCATGAGTGTGTCTTCCCGTTTTCCGCCTGAGGTCACTGCGTGGATGGAGCGCTGGCGCCTGCTGCGCGACGGCGAGCTGCTCACCACCCACTCGAGCTGGATACTTCCCGTCCGCCAGGGGGACATGCCGGCGATGCTGAAGGTCGCGCGCATTCCCGATGAAGAGGCCGGTTACCGCCTGTTGACCTGGTGGGACGGGCAGGGCGCCGCCCGAGTCTTCGCCTCGGCGGCGGGCGCTCTGCTCATGGAGCGCGCGTCCGGGGCCGGGGACCTTGCACAGATAGCGTGGTCCGGCCAGGACGACGAGGCTTGCAGGATCCTCTGCGACACCGCCGCTCGTCTGCACGCGCCGCGGTCCGGACCGCCGCCCGATCTCCATCCGCTACAGGAATGGTTCCAGCCGCTTTTCCGGTTGGCCGCTGAGCACGCGGCACTTGCGCCCGCCGCCAGCGTAGCGCGCCAACTTCTGGCGGCGCCGCGCGAGGTGTGCCCGCTCCACGGCGACCTGCACCACGAGAACGTGCTCGACTTCGGCGACCGCGGCTGGCTGGCCATCGACCCGCACGGACTGCTCGGCGAGCGCACCTTCGACTATGCCAACATCTTCACGAATCCCGATCTCAGCGACCCCGGTCGCCCGCTTGCGATCCTGCCGGGCAGGCTGGAGGCTCGACTCAGCATTGTGGTCGCGACGACCGGGTTTGAGCCCGAACGGCTTCTTCGCTGGATCATTGCATGGACGGGCTTGTCGGCAGCCTGGTTCATCGGCGACGGCGACGGCGAGGGCGAGGGCGCTGCGATTGATCTGGCCGTAAACGCCATGGCACGCCGGTTGCTTGACTAGCGCGGTCACCGATCTCACCTGGTCGTCGAGCTAGGTCAGGCCGTGTCGGGCGTGATCCGCTGGAAGTCGTTGCGGGCCACACCCGCCGCCTCGAAGCCCTGCACCAGGCCGGCATCGTGGTGTGCGTGGCCGAGGGACTATGGAAGGTGCCGGACGATCTGCCCGAGCAGGGCCGCCGCTATGACGCCCAGCGTCTTGGTGGCGTGACGGTGGAGCTGAAATCGCACCTGCCCATCGAGCGGCAGGCCCGCGTGATCGGTGCCACCTGGCTTGACCAGCAGTTGATCGACGGTGGCTCGGGCTTGGGCGACCTGGGCTTTAGCAGTGAGGCCAAGTAGGCGATACAGCAGCGCGCGGACTTCCTGGCCGAACAGGGACTGGCCGAGCGGCGCGGGCAGCGCGTGATCCTCACCGGAATCTGCTCGGCAGCAGCGGGCTCGGGAACTGGCGCAGGCCGCGAAGGACATTGCCGCCGATACCGGCCTGGAGCATCGCCCCGTGGCCGACGGCCAGCGCGTTGCCGGCGTCTACCGGCGCCCCGTCATGCTCGCCAGCGGGCGAAATGGGATGCTTGATGACGCCAAGGGGTCCAGCCTCGTGCGGTGGAAGCCCATCGAACAGCGGCTTGGGGAGCAGCTCGCCGCGACGGTGCGCGGTGGCGGCGTGTCTTGGGAGATTGGACGACAGCGTGGGCCGGCCCCTGTCTCTTGATCAGATCTTGATCCCCTGCGCCATCAGATCCTTGGCGGCAAGAAAGCCATCCAGTTTACTTTGCAGGGCTTCCCAACCTTCCCAGAGGGCGCCCCAGCTGGCAATTCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAAGCTTTCTCTGAGCTGTAACAGCCTGACCGCAACAAACGAGAGGATCGAGACCATCCGCTCCAGATTATCCGGCTCCTCCATGCGTTGCCTCTCGGCTCCTGCTCCGGTTTTCCATGCCTTATGGAACTCCTCGATCCGCCAGCGATGGGTATAAATGTCGATGACGCGCAAGGCTTGGGCTAGCGACTCGACCGGTTCGCCGGTCAGCAACAACCATTTCAACGGGGTCTCACCCTTGGGCGGGTTAATCTCCTCGGCCAGCACCGCGTTGAGCGTGATATTCCCCTGTTTTAGCGTGATGCGCCCACTGCGCAGGCTCAAGCTCGCCTTGCGGGCTGGTCGATTTTTACGTTTACCGCGTTTATCCACCACGCCCTTTTGCGGAATGCTGATCTGATAGCCACCCAACTCCGGTTGGTTCTTCAGATGGTCGATCAGATACAACCCAGACTCTACGTCCTTGCGTGGGTGCTTGGAGCGCACCACGAAGCGCTCGTTATGCGCCAGCCTGTCCTGCAGATAAGCATGAATATCGGCTTCGCGGTCACAGACCGCAATCACGTTGCTCATCATGCTGCCCATGCGTAACCGGCTAGTTGCGGCCGCTGCCAGCCATTTGCCACTCTCCTTTTCATCCGCATCGGCAGGGTCATCCGGGCGCATCCACCACTCCTGATGCAGTAATCCTACGGTGCGGAATGTGGTGGCCTCGAGCAAGAGAACGGAGTGAACCCACCATCCGCGGGATTTATCCTGAATAGAGCCCAGCTTGCCAAGCTCTTCGGCGACCTGGTGGCGATAACTCAAAGAGGTGGTGTCCTCAATGGCCAGCAGTTCGGGAAACTCCTGAGCCAACTTGACTGTTTGCATGGCGCCAGCCTTTCTGATCGCCTCGGCAGAAACGTTGGGATTGCGGTAAAATCGGTAAGCGCCTTCCTGCATGGCTTCACTACCCTCTGATGAGATGGTTATTGATTTACCAGAATATTTTGCCAATTGGGCGGCGACGTTAACCAAGCGGGCAGTACGGCGAGGATCACCCAGCGCCGCCGAAGAGAACACAGATTTAGCCCAGTCGGCCGCACGATGAAGAGCAGAAGTTATCATGAACGTTACCATGTTAGGAGGTCACATGGAAGTCAGATCCTGGAAAACGGGAAAGGTTCCGTTCAGGACGCTACTTGTGTATAAGAGTCAG
   3 PRIMER_PAIR_PENALTY=0.0034
   4 PRIMER_LEFT_PENALTY=0.000037
   5 PRIMER_RIGHT_PENALTY=0.003322
   6 PRIMER_LEFT_SEQUENCE=GACCGAAAGCAACGTACCAT
   7 PRIMER_RIGHT_SEQUENCE=ATGACCAGCGTGTTACCCTC
   8 PRIMER_LEFT=846,20
   9 PRIMER_RIGHT=1131,20
  10 PRIMER_LEFT_TM=60.000
  11 PRIMER_RIGHT_TM=59.997
  12 PRIMER_LEFT_GC_PERCENT=50.000
  13 PRIMER_RIGHT_GC_PERCENT=55.000
  14 PRIMER_LEFT_SELF_ANY=4.00
  15 PRIMER_RIGHT_SELF_ANY=3.00
  16 PRIMER_LEFT_SELF_END=2.00
  17 PRIMER_RIGHT_SELF_END=3.00
  18 PRIMER_LEFT_END_STABILITY=7.8000
  19 PRIMER_RIGHT_END_STABILITY=9.4000
  20 PRIMER_PAIR_COMPL_ANY=4.00
  21 PRIMER_PAIR_COMPL_END=1.00
  22 PRIMER_PRODUCT_SIZE=286
  23 PRIMER_PAIR_PENALTY_1=0.0180
  24 PRIMER_LEFT_1_PENALTY=0.003322
  25 PRIMER_RIGHT_1_PENALTY=0.014712
  26 PRIMER_LEFT_1_SEQUENCE=GAGGGTAACACGCTGGTCAT
  27 PRIMER_RIGHT_1_SEQUENCE=GAATCCTCTTCGGAGTTCCC
  28 PRIMER_LEFT_1=1112,20
  29 PRIMER_RIGHT_1=1263,20
  30 PRIMER_LEFT_1_TM=59.997
  31 PRIMER_RIGHT_1_TM=60.015
  32 PRIMER_LEFT_1_GC_PERCENT=55.000
  33 PRIMER_RIGHT_1_GC_PERCENT=55.000
  34 PRIMER_LEFT_1_SELF_ANY=3.00
  35 PRIMER_RIGHT_1_SELF_ANY=4.00
  36 PRIMER_LEFT_1_SELF_END=2.00
  37 PRIMER_RIGHT_1_SELF_END=2.00
  38 PRIMER_LEFT_1_END_STABILITY=6.3000
  39 PRIMER_RIGHT_1_END_STABILITY=9.7000
  40 PRIMER_PAIR_1_COMPL_ANY=4.00
  41 PRIMER_PAIR_1_COMPL_END=1.00
  42 PRIMER_PRODUCT_SIZE_1=152
  43 PRIMER_PAIR_PENALTY_2=0.0213
  44 PRIMER_LEFT_2_PENALTY=0.000037
  45 PRIMER_RIGHT_2_PENALTY=0.021275
  46 PRIMER_LEFT_2_SEQUENCE=GACCGAAAGCAACGTACCAT
  47 PRIMER_RIGHT_2_SEQUENCE=CTTTTTAGCGAATTCCAGCG
  48 PRIMER_LEFT_2=846,20
  49 PRIMER_RIGHT_2=1111,20
  50 PRIMER_LEFT_2_TM=60.000
  51 PRIMER_RIGHT_2_TM=59.979
  52 PRIMER_LEFT_2_GC_PERCENT=50.000
  53 PRIMER_RIGHT_2_GC_PERCENT=45.000
  54 PRIMER_LEFT_2_SELF_ANY=4.00
  55 PRIMER_RIGHT_2_SELF_ANY=6.00
  56 PRIMER_LEFT_2_SELF_END=2.00
  57 PRIMER_RIGHT_2_SELF_END=2.00
  58 PRIMER_LEFT_2_END_STABILITY=7.8000
  59 PRIMER_RIGHT_2_END_STABILITY=10.2000
  60 PRIMER_PAIR_2_COMPL_ANY=4.00
  61 PRIMER_PAIR_2_COMPL_END=0.00
  62 PRIMER_PRODUCT_SIZE_2=266
  63 PRIMER_PAIR_PENALTY_3=0.0354
  64 PRIMER_LEFT_3_PENALTY=0.019325
  65 PRIMER_RIGHT_3_PENALTY=0.016052
  66 PRIMER_LEFT_3_SEQUENCE=AGACAATCGGCTGCTCTGAT
  67 PRIMER_RIGHT_3_SEQUENCE=CTCGTCCTGCAGTTCATTCA
  68 PRIMER_LEFT_3=3544,20
  69 PRIMER_RIGHT_3=3653,20
  70 PRIMER_LEFT_3_TM=59.981
  71 PRIMER_RIGHT_3_TM=59.984
  72 PRIMER_LEFT_3_GC_PERCENT=50.000
  73 PRIMER_RIGHT_3_GC_PERCENT=50.000
  74 PRIMER_LEFT_3_SELF_ANY=4.00
  75 PRIMER_RIGHT_3_SELF_ANY=6.00
  76 PRIMER_LEFT_3_SELF_END=3.00
  77 PRIMER_RIGHT_3_SELF_END=1.00
  78 PRIMER_LEFT_3_END_STABILITY=6.6000
  79 PRIMER_RIGHT_3_END_STABILITY=6.9000
  80 PRIMER_PAIR_3_COMPL_ANY=4.00
  81 PRIMER_PAIR_3_COMPL_END=2.00
  82 PRIMER_PRODUCT_SIZE_3=110
  83 PRIMER_PAIR_PENALTY_4=0.0354
  84 PRIMER_LEFT_4_PENALTY=0.019325
  85 PRIMER_RIGHT_4_PENALTY=0.016052
  86 PRIMER_LEFT_4_SEQUENCE=AGACAATCGGCTGCTCTGAT
  87 PRIMER_RIGHT_4_SEQUENCE=TCGTCCTGCAGTTCATTCAG
  88 PRIMER_LEFT_4=3544,20
  89 PRIMER_RIGHT_4=3652,20
  90 PRIMER_LEFT_4_TM=59.981
  91 PRIMER_RIGHT_4_TM=59.984
  92 PRIMER_LEFT_4_GC_PERCENT=50.000
  93 PRIMER_RIGHT_4_GC_PERCENT=50.000
  94 PRIMER_LEFT_4_SELF_ANY=4.00
  95 PRIMER_RIGHT_4_SELF_ANY=6.00
  96 PRIMER_LEFT_4_SELF_END=3.00
  97 PRIMER_RIGHT_4_SELF_END=2.00
  98 PRIMER_LEFT_4_END_STABILITY=6.6000
  99 PRIMER_RIGHT_4_END_STABILITY=7.0000
 100 PRIMER_PAIR_4_COMPL_ANY=4.00
 101 PRIMER_PAIR_4_COMPL_END=3.00
 102 PRIMER_PRODUCT_SIZE_4=109
 103 =