1 LOCUS PNECG 2355 bp DNA circular BCT 21-APR-1996
2 DEFINITION Plasmid pNE131 (from S.epidermidis), complete genome.
4 VERSION M12730.1 GI:150829
5 KEYWORDS MLS antibiotics resistance; complete genome;
6 macrolide-lincosamide-streptogramin B resistance; plasmid;
7 replication and maintenance protein.
9 ORGANISM Plasmid pNE131
10 other sequences; plasmids; Plasmid.
11 REFERENCE 1 (bases 696 to 1586)
12 AUTHORS Lampson,B.C. and Parisi,J.T.
13 TITLE Naturally occurring Staphylococcus epidermidis plasmid expressing
14 constitutive macrolide-lincosamide-streptogramin B resistance
15 contains a deleted attenuator
16 JOURNAL J. Bacteriol. 166 (2), 479-483 (1986)
18 REFERENCE 2 (bases 1 to 2355)
19 AUTHORS Lampson,B.C. and Parisi,J.T.
20 TITLE Nucleotide sequence of the constitutive
21 macrolide-lincosamide-streptogramin B resistance plasmid pNE131
22 from Staphylococcus epidermidis and homologies with Staphylococcus
23 aureus plasmids pE194 and pSN2
24 JOURNAL J. Bacteriol. 167 (3), 888-892 (1986)
26 COMMENT Original source text: Plasmid pNE131 (clone: pNE131.) DNA. Draft
27 entry and clean copy sequence for [2] kindly provided by
28 J.T.Parisi, 04-AUG-1986. Ribosome binding sites are located at
29 positions 754-759, 769-773, and 1944-1948; a -35 region at
30 1889-1899, and -10 regions at 733-738, and 1927-1932.
31 FEATURES Location/Qualifiers
34 /mol_type="genomic DNA"
36 /specific_host="Staphylococcus epidermidis"
37 /plasmid="Plasmid pNE131"
38 /organism="Plasmid pNE131"
39 CDS join(1959..2355,1..92)
42 /protein_id="AAA98295.1"
43 /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
44 QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
45 GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALYLIIILSRTS
47 /product="replication and maintenance protein"
58 /protein_id="AAA98296.1"
63 /product="macrolide-lincosamide-streptogramin
65 /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
66 TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
67 PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSFALFLMAEVDISILSMVPR
68 EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
69 AGIDDLNNISFEQFLSLFNSYKLFNK"
73 BASE COUNT 911 a 359 c 371 g 714 t
74 ORIGIN 1 bp upstream of TaqI site.
75 1 tcgaatttgg gaactttgag caagaggcaa atgaaaaaca agaaaatgca ctttatctga
76 61 ttattattct ttcaaggact agtataacat aaatcgtcta caaatagaca aaaaacctgc
77 121 acgcttaatg tagatcaaaa gcttaacgca aatgaaatag attgacctcc caataacacc
78 181 acgttagtta ttgggagtca atctatgaaa tgcgattaag ctttttctaa ttcacataag
79 241 cgtgcaggtt taaagtacat aaaaaatata atgaaaaaaa gcatcattat actaacgtta
80 301 taccaacatt atactaattg cttattccaa tttcctattg gttggaacca acaggcgtta
81 361 gtgtgttgtt gagttggtac tttcatggga ttaatcccat gaaaccccca accaactcgc
82 421 caaagctttg gctaacacac acgccattcc aaccaatagt tttctcggca ttaaagccat
83 481 gctctgacgc ttaaatgcac taatgcctta aaaaaacatt aaagtctaac acactagact
84 541 tattttcatt cgtaattaag tcgttaaacc gtgtgctcta cgaccaaaag tataaaacct
85 601 ttaagaactt tcttttttct tgtaaaaaaa gaaactagat aaatctctca tatcttttat
86 661 tcaataatcg catcagattg cagtataaat ttaacgatca ctcatcatgt tcatatttat
87 721 cagagctcgt gctataatta tactaatttt ataaggagga aaaaataaag agggttataa
88 781 tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat aatatagata
89 841 aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc ggctcaggaa
90 901 aagggcattt tacccttgaa ttagtacaga ggtgtaattt cgtaactgcc attgaaatag
91 961 accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat ttccaagttt
92 1021 taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa atatttggta
93 1081 atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat agtatagctg
94 1141 atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat acaaaacgct
95 1201 cattcgcatt atttttaatg gcagaagttg atatttctat attaagtatg gttccaagag
96 1261 aatattttca tcctaaacct aaagtgaata gctcacttat cagattaaat agaaaaaaat
97 1321 caagaatatc acacaaagat aaacagaagt ataattattt cgttatgaaa tgggttaaca
98 1381 aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa catgcaggaa
99 1441 ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat agctataaat
100 1501 tatttaataa gtaagttaag ggatgcataa actgcatcct taacttgttt ttcgtgtacc
101 1561 tattttttgt gaatcgatta tgtcttttgc gcattcactt cttttctata taaatatgag
102 1621 cgacagataa gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg
103 1681 ttcagggggt gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt
104 1741 acgttagata accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg
105 1801 taaaatctta atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt
106 1861 tcactcattt tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga
107 1921 tatagttaga atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag
108 1981 tctataaagg ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca
109 2041 agttataccg taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa
110 2101 gtatgttaga tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg
111 2161 tccacttaag taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa
112 2221 caagtctaca aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa
113 2281 gaaaaactgg agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac
114 2341 aaaaatacct cttac