1 TBLASTX 2.0MP-WashU [12-Feb-2001] [linux-i686 01:36:08 31-Jan-2001]
3 Copyright (C) 1996-2000 Washington University, Saint Louis, Missouri USA.
6 Reference: Gish, W. (1996-2000) http://blast.wustl.edu
8 Notice: statistical significance is estimated under the assumption that the
9 equivalent of one entire reading frame of the query sequence and one entire
10 reading frame of the database code for protein and that significant alignments
11 will only involve coding reading frames.
13 Query= gi|142864|gb|M10040.1|BACDNAE B.subtilis dnaE gene encoding DNA
17 Translating both strands of query sequence in all 6 reading frames
20 400 sequences; 4,662,239 total letters.
21 Searching....10....20....30....40....50....60....70....80....90....100% done
25 Reading High Probability
26 Sequences producing High-scoring Segment Pairs: Frame Score P(N) N
28 gi|1789441|gb|AE000388.1|AE000388 Escherichia coli K-1... +2 318 6.4e-70 5
29 gi|2367383|gb|AE000509.1|AE000509 Escherichia coli K-1... -3 59 0.9992 2
33 >gi|1789441|gb|AE000388.1|AE000388 Escherichia coli K-12 MG1655 section 278 of
34 400 of the complete genome
39 Score = 318 (148.6 bits), Expect = 6.4e-70, Sum P(5) = 6.4e-70
40 Identities = 57/127 (44%), Positives = 81/127 (63%), Frame = +3 / +2
42 Query: 618 FDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETPLFHKSKLLYNFYKARLHIR 797
43 +DRFR RVMFPI D G V+ F GR LG+ PKY+NSPET +FHK + LY Y+A+
44 Sbjct: 5366 YDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNA 5545
46 Query: 798 KQERAVLFEGFADVYTAVSSDVKESIATMGTSLTDDHVKILRRNVEEIILCYDSDKAGYE 977
47 + R ++ EG+ DV + ++A++GTS T DH+++L R +I CYD D+AG +
48 Sbjct: 5546 EPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRD 5725
50 Query: 978 ATLKASE 998
52 Sbjct: 5726 AAWRALE 5746
54 Score = 247 (116.1 bits), Expect = 6.4e-70, Sum P(5) = 6.4e-70
55 Identities = 44/98 (44%), Positives = 61/98 (62%), Frame = +3 / +2
57 Query: 21 MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH 200
58 M RIP ++ + DIV++I V+LKKQG+N+ CPFH E TPSF+V+ +KQ +H
59 Sbjct: 4778 MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYH 4957
61 Query: 201 CFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFP 314
62 CFGCGA GN FL + F E+V LA + ++ P
63 Sbjct: 4958 CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVP 5071
65 Score = 85 (41.8 bits), Expect = 6.4e-70, Sum P(5) = 6.4e-70
66 Identities = 19/59 (32%), Positives = 28/59 (47%), Frame = +3 / +2
68 Query: 441 ALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGFSEAQMEKAGLLIRREDGSGY 617
69 A YL RG + E+I F IG+A WD + K + + AG+L+ + G Y
70 Sbjct: 5192 ARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSY 5368
72 Score = 65 (32.7 bits), Expect = 6.4e-70, Sum P(5) = 6.4e-70
73 Identities = 12/28 (42%), Positives = 18/28 (64%), Frame = +3 / +2
75 Query: 1014 GCKVRVAMIPDGLDPDDYIKKFGGEKFK 1097
76 G ++R +PDG DPD ++K G E F+
77 Sbjct: 5771 GRQLRFMFLPDGEDPDTLVRKEGKEAFE 5854
79 Score = 48 (24.9 bits), Expect = 0.89, Sum P(4) = 0.59
80 Identities = 15/49 (30%), Positives = 21/49 (42%), Frame = +3 / +2
82 Query: 639 VMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETPLFHKSKLLYNFYKAR 785
83 V F HD V A R+ + P Y T LFH+ K F++ +
84 Sbjct: 4046 VRFYAHDRGRPVQARRRRSQRAISPDYTIGWLTNLFHRRKWRCRFHRRK 4192
86 Score = 46 (24.0 bits), Expect = 1.5, Sum P(4) = 0.78
87 Identities = 8/17 (47%), Positives = 10/17 (58%), Frame = +3 / +2
89 Query: 150 GESTPSFSVSPDKQIFH 200
91 Sbjct: 5447 GNDTPKYLNSPETDIFH 5497
93 Score = 42 (22.1 bits), Expect = 6.4e-70, Sum P(5) = 6.4e-70
94 Identities = 6/16 (37%), Positives = 10/16 (62%), Frame = +1 / +2
96 Query: 1129 WRSKCNISEKERTCPM 1176
98 Sbjct: 7151 WNSTIVLKQKKRVCPI 7198
100 Score = 42 (22.1 bits), Expect = 2.0e-42, Sum P(5) = 2.0e-42
101 Identities = 6/18 (33%), Positives = 14/18 (77%), Frame = +3 / +2
103 Query: 21 MGNRIPDEIVDQVQKSAD 74
105 Sbjct: 206 ISTRMPDDVVDKLKQLKD 259
109 Score = 102 (49.6 bits), Expect = 4.9e-08, Sum P(3) = 4.9e-08
110 Identities = 32/83 (38%), Positives = 42/83 (50%), Frame = -3 / -3
112 Query: 319 SSGKSIWYLSAR*ETDSAKE*PSICLKKEKTLPPAPQPKQ*KICLSGDTENEGVLSP*KG 140
113 S+G S ++A T S S ++K LP AP PKQ C S T +GV S G
114 Sbjct: 5076 SNGTSRLCIAASSSTVSTNSSLS*FIRKSIALPCAPHPKQW*NCFSPLTVKDGVFSLWNG 4897
116 Query: 139 QSPK*FRPCFFN*T*SPMTSTIS 71
118 Sbjct: 4896 QHAWKFLPCFFSFTRASIRSTMS 4828
120 Score = 71 (35.4 bits), Expect = 4.9e-08, Sum P(3) = 4.9e-08
121 Identities = 18/35 (51%), Positives = 20/35 (57%), Frame = -3 / -3
123 Query: 724 FIYLGCWLPRALPEKATTAP**SWIGNMTRFLKRS 620
124 F YLG LP P K T P S +GN+TR KRS
125 Sbjct: 5472 FRYLGVSLPSTRPPKPITRPRLSRMGNITRSRKRS 5368
127 Score = 65 (32.7 bits), Expect = 0.0034, Sum P(3) = 0.0034
128 Identities = 18/71 (25%), Positives = 34/71 (47%), Frame = -2 / -2
130 Query: 233 ENIAARSAAKAMKNLFVGRYGKRRCAFSMKRTESKVVSALLL*LNIITYDFNDICRLLHL 54
131 + +A R+ +KA+ LF+ G+ R ++ T + ALL L+ +D+ +
132 Sbjct: 4990 DRVAVRATSKAVVKLFLTVNGEGRGFLVVEWTTRVEILALLFQLHTGIDQIDDVSACQQI 4811
134 Query: 53 IHNFIWYPISH 21
136 Sbjct: 4810 INEYAWDSSSH 4778
138 Score = 42 (22.1 bits), Expect = 4.9e-08, Sum P(3) = 4.9e-08
139 Identities = 10/18 (55%), Positives = 12/18 (66%), Frame = -3 / -3
141 Query: 1093 NFSPPNFLM*SSGSNPSG 1040
143 Sbjct: 5850 NASLPSFRTSVSGSSPSG 5797
146 >gi|2367383|gb|AE000509.1|AE000509 Escherichia coli K-12 MG1655 section 399 of
147 400 of the complete genome
152 Score = 59 (29.9 bits), Expect = 7.1, Sum P(2) = 1.00
153 Identities = 15/40 (37%), Positives = 19/40 (47%), Frame = -3 / -3
155 Query: 706 WLPRALPEKATTAP**SWIGNMTRFLKRSKYPLPSSRLIR 587
156 WL R +T +P WI M L SK LPS++ R
157 Sbjct: 4227 WLSRTTVGSSTVSPRTFWITRMKVKLSSSKVTLPSTKSTR 4108
159 Score = 51 (26.3 bits), Expect = 7.1, Sum P(2) = 1.00
160 Identities = 8/15 (53%), Positives = 10/15 (66%), Frame = -3 / -1
162 Query: 382 CASAIFCSPEDSGRA 338
164 Sbjct: 1097 CTSLMLCRPQDGGRA 1053
191 Query ----- As Used ----- ----- Computed ----
192 Frame MatID Matrix name Lambda K H Lambda K H
193 +3 0 blosum62 0.318 0.135 0.401 0.324 0.139 0.405
194 +2 0 blosum62 0.318 0.135 0.401 0.365 0.163 0.618
195 +1 0 blosum62 0.318 0.135 0.401 0.356 0.155 0.528
196 -1 0 blosum62 0.318 0.135 0.401 0.350 0.155 0.543
197 -2 0 blosum62 0.318 0.135 0.401 0.350 0.155 0.505
198 -3 0 blosum62 0.318 0.135 0.401 0.358 0.157 0.543
201 Frame MatID Length Eff.Length E S W T X E2 S2
202 +3 0 666 666 10. 54 3 13 16 0.021 41
203 +2 0 666 666 10. 54 3 13 16 0.021 41
204 +1 0 667 667 10. 54 3 13 16 0.021 41
205 -1 0 667 667 10. 54 3 13 16 0.021 41
206 -2 0 666 666 10. 54 3 13 16 0.021 41
207 -3 0 666 666 10. 54 3 13 16 0.021 41
212 Database: /home/jes12/db/ecoli.nt
214 Posted: 3:16:20 PM EST Nov 18, 2001
215 Created: 10:10:31 AM EST Nov 18, 2001
217 # of letters in database: 4,662,239
218 # of sequences in database: 400
219 # of database sequences satisfying E: 2
220 No. of states in DFA: 598 (64 KB)
221 Total size of DFA: 416 KB (1196 KB)
222 Time to generate neighborhood: 0.02u 0.02s 0.04t Elapsed: 00:00:00
223 No. of threads or processors used: 1
224 Search cpu time: 2.41u 0.01s 2.42t Elapsed: 00:00:05
225 Total cpu time: 2.44u 0.03s 2.47t Elapsed: 00:00:07
226 Start: Sat Apr 20 18:19:43 2002 End: Sat Apr 20 18:19:50 2002