Bio/Search/Hit/ModelHit.pm

   1 #
   2 # BioPerl module for Bio::Search::Hit::ModelHit
   3 #
   4 # Please direct questions and support issues to <bioperl-l@bioperl.org>
   5 #
   6 # Cared for by Chris Fields <cjfields at bioperl dot org>
   7 #
   8 # Copyright Chris Fields
   9 #
  10 # You may distribute this module under the same terms as perl itself
  11
  12 # POD documentation - main docs before the code
  13
  14 =head1 NAME
  15
  16 Bio::Search::Hit::ModelHit - A model-based implementation of the Bio::Search::Hit::HitI interface
  17
  18 =head1 SYNOPSIS
  19
  20     use Bio::Search::Hit::ModelHit;
  21     my $hit = Bio::Search::Hit::ModelHit->new(-algorithm => 'rnamotif');
  22
  23     # typically one gets HitI objects from a SearchIO stream via a ResultI
  24     use Bio::SearchIO;
  25     my $parser = Bio::SearchIO->new(-format => 'infernal', -file => 'trap.inf');
  26
  27     my $result = $parser->next_result;
  28     my $hit    = $result->next_hit;
  29
  30 =head1 DESCRIPTION
  31
  32 This object handles the hit data from a database search using models or
  33 descriptors instead of sequences, such as Infernal, HMMER, RNAMotif, etc.
  34
  35 Unless you're writing a parser, you won't ever need to create a ModelHit or
  36 any other HitI-implementing object. If you use the SearchIO system, HitI objects
  37 are created automatically from a SearchIO stream which returns
  38 Bio::Search::Hit::HitI objects.
  39
  40 Note that several HitI-based methods have been overridden from ModelHit due to
  41 their unreliability when dealing with queries that aren't sequence-based. It may
  42 be possible to reimplement these at a later point, but for the time being they
  43 will throw warnings and return w/o results.
  44
  45 For documentation on what you can do with ModelHit (and other HitI objects),
  46 please see the API documentation in
  47 L<Bio::Search::Hit::HitI|Bio::Search::Hit::HitI>.
  48
  49 =head1 FEEDBACK
  50
  51 =head2 Mailing Lists
  52
  53 User feedback is an integral part of the evolution of this and other
  54 Bioperl modules. Send your comments and suggestions preferably to
  55 the Bioperl mailing list.  Your participation is much appreciated.
  56
  57   bioperl-l@bioperl.org                  - General discussion
  58   http://bioperl.org/wiki/Mailing_lists  - About the mailing lists
  59
  60 =head2 Support
  61
  62 Please direct usage questions or support issues to the mailing list:
  63
  64 I<bioperl-l@bioperl.org>
  65
  66 rather than to the module maintainer directly. Many experienced and
  67 reponsive experts will be able look at the problem and quickly
  68 address it. Please include a thorough description of the problem
  69 with code and data examples if at all possible.
  70
  71 =head2 Reporting Bugs
  72
  73 Report bugs to the Bioperl bug tracking system to help us keep track
  74 of the bugs and their resolution. Bug reports can be submitted via the
  75 web:
  76
  77   https://github.com/bioperl/bioperl-live/issues
  78
  79 =head1 AUTHOR - Chris Fields
  80
  81 Email cjfields at bioperl dot org
  82
  83 =head1 APPENDIX
  84
  85 The rest of the documentation details each of the object methods.
  86 Internal methods are usually preceded with a _
  87
  88 =cut
  89
  90 # Let the code begin...
  91
  92 package Bio::Search::Hit::ModelHit;
  93
  94 use strict;
  95
  96 use base qw(Bio::Search::Hit::GenericHit);
  97
  98 =head1 HitI methods implemented in parent class Bio::Search::Hit::ModelHit
  99
 100 =head2 new
 101
 102  Title   : new
 103  Usage   : my $obj = Bio::Search::Hit::ModelHit->new();
 104  Function: Builds a new Bio::Search::Hit::ModelHit object
 105  Returns : Bio::Search::Hit::ModelHit
 106  Args    : -name         => Name of Hit (required)
 107            -description  => Description (optional)
 108            -accession    => Accession number (optional)
 109            -ncbi_gi      => NCBI GI UID (optional)
 110            -length       => Length of the Hit (optional)
 111            -score        => Raw Score for the Hit (optional)
 112            -bits         => Bit Score for the Hit (optional)
 113            -significance => Significance value for the Hit (optional)
 114            -algorithm    => Algorithm used (BLASTP, FASTX, etc...)
 115            -hsps         => Array ref of HSPs for this Hit.
 116            -found_again  => boolean, true if hit appears in a
 117                             "previously found" section of a PSI-Blast report.
 118            -hsp_factory  => Bio::Factory::ObjectFactoryI able to create HSPI
 119                             objects.
 120
 121 =cut
 122
 123 =head2 add_hsp
 124
 125  Title   : add_hsp
 126  Usage   : $hit->add_hsp($hsp)
 127  Function: Add a HSP to the collection of HSPs for a Hit
 128  Returns : number of HSPs in the Hit
 129  Args    : Bio::Search::HSP::HSPI object, OR hash ref containing data suitable
 130            for creating a HSPI object (&hsp_factory must be set to get it back)
 131
 132 =cut
 133
 134 =head2 hsp_factory
 135
 136  Title   : hsp_factory
 137  Usage   : $hit->hsp_factory($hsp_factory)
 138  Function: Get/set the factory used to build HSPI objects if necessary.
 139  Returns : Bio::Factory::ObjectFactoryI
 140  Args    : Bio::Factory::ObjectFactoryI
 141
 142 =cut
 143
 144 =head2 Bio::Search::Hit::HitI methods
 145
 146 Implementation of Bio::Search::Hit::HitI methods
 147
 148 =head2 name
 149
 150  Title   : name
 151  Usage   : $hit_name = $hit->name();
 152  Function: returns the name of the Hit sequence
 153  Returns : a scalar string
 154  Args    : [optional] scalar string to set the name
 155
 156 =cut
 157
 158 =head2 accession
 159
 160  Title   : accession
 161  Usage   : $acc = $hit->accession();
 162  Function: Retrieve the accession (if available) for the hit
 163  Returns : a scalar string (empty string if not set)
 164  Args    : none
 165
 166 =cut
 167
 168 =head2 description
 169
 170  Title   : description
 171  Usage   : $desc = $hit->description();
 172  Function: Retrieve the description for the hit
 173  Returns : a scalar string
 174  Args    : [optional] scalar string to set the descrition
 175
 176 =cut
 177
 178 =head2 length
 179
 180  Title   : length
 181  Usage   : my $len = $hit->length
 182  Function: Returns the length of the hit
 183  Returns : integer
 184  Args    : [optional] integer to set the length
 185
 186 =cut
 187
 188 =head2 algorithm
 189
 190  Title   : algorithm
 191  Usage   : $alg = $hit->algorithm();
 192  Function: Gets the algorithm specification that was used to obtain the hit
 193            For BLAST, the algorithm denotes what type of sequence was aligned
 194            against what (BLASTN: dna-dna, BLASTP prt-prt, BLASTX translated
 195            dna-prt, TBLASTN prt-translated dna, TBLASTX translated
 196            dna-translated dna).
 197  Returns : a scalar string
 198  Args    : [optional] scalar string to set the algorithm
 199
 200 =cut
 201
 202 =head2 raw_score
 203
 204  Title   : raw_score
 205  Usage   : $score = $hit->raw_score();
 206  Function: Gets the "raw score" generated by the algorithm.  What
 207            this score is exactly will vary from algorithm to algorithm,
 208            returning undef if unavailable.
 209  Returns : a scalar value
 210  Args    : [optional] scalar value to set the raw score
 211
 212 =cut
 213
 214 =head2 score
 215
 216 Equivalent to L<raw_score()|raw_score>
 217
 218 =cut
 219
 220 =head2 significance
 221
 222  Title   : significance
 223  Usage   : $significance = $hit->significance();
 224  Function: Used to obtain the E or P value of a hit, i.e. the probability that
 225            this particular hit was obtained purely by random chance.  If
 226            information is not available (nor calculatable from other
 227            information sources), return undef.
 228  Returns : a scalar value or undef if unavailable
 229  Args    : [optional] scalar value to set the significance
 230
 231 =cut
 232
 233 =head2 bits
 234
 235  Usage     : $hit_object->bits();
 236  Purpose   : Gets the bit score of the best HSP for the current hit.
 237  Example   : $bits = $hit_object->bits();
 238  Returns   : Integer or undef if bit score is not set
 239  Argument  : n/a
 240  Comments  : For BLAST1, the non-bit score is listed in the summary line.
 241
 242 See Also   : L<score()|score>
 243
 244 =cut
 245
 246 =head2 next_hsp
 247
 248  Title    : next_hsp
 249  Usage    : while( $hsp = $obj->next_hsp()) { ... }
 250  Function : Returns the next available High Scoring Pair
 251  Example  :
 252  Returns  : Bio::Search::HSP::HSPI object or null if finished
 253  Args     : none
 254
 255 =cut
 256
 257 =head2 hsps
 258
 259  Usage     : $hit_object->hsps();
 260  Purpose   : Get a list containing all HSP objects.
 261            : Get the numbers of HSPs for the current hit.
 262  Example   : @hsps = $hit_object->hsps();
 263            : $num  = $hit_object->hsps();  # alternatively, use num_hsps()
 264  Returns   : Array context : list of Bio::Search::HSP::BlastHSP.pm objects.
 265            : Scalar context: integer (number of HSPs).
 266            :                 (Equivalent to num_hsps()).
 267  Argument  : n/a. Relies on wantarray
 268  Throws    : Exception if the HSPs have not been collected.
 269
 270 See Also   : L<hsp()|hsp>, L<num_hsps()|num_hsps>
 271
 272 =cut
 273
 274 =head2 num_hsps
 275
 276  Usage     : $hit_object->num_hsps();
 277  Purpose   : Get the number of HSPs for the present hit.
 278  Example   : $nhsps = $hit_object->num_hsps();
 279  Returns   : Integer or '-' if HSPs have not been callected
 280  Argument  : n/a
 281
 282 See Also   : L<hsps()|hsps>
 283
 284 =cut
 285
 286 =head2 rewind
 287
 288  Title   : rewind
 289  Usage   : $hit->rewind;
 290  Function: Allow one to reset the HSP iterator to the beginning
 291            Since this is an in-memory implementation
 292  Returns : none
 293  Args    : none
 294
 295 =cut
 296
 297 =head2 ambiguous_aln
 298
 299  Usage     : $ambig_code = $hit_object->ambiguous_aln();
 300  Purpose   : Sets/Gets ambiguity code data member.
 301  Example   : (see usage)
 302  Returns   : String = 'q', 's', 'qs', '-'
 303            :   'q'  = query sequence contains overlapping sub-sequences
 304            :          while sbjct does not.
 305            :   's'  = sbjct sequence contains overlapping sub-sequences
 306            :          while query does not.
 307            :   'qs' = query and sbjct sequence contains overlapping sub-sequences
 308            :          relative to each other.
 309            :   '-'  = query and sbjct sequence do not contains multiple domains
 310            :          relative to each other OR both contain the same distribution
 311            :          of similar domains.
 312  Argument  : n/a
 313  Throws    : n/a
 314  Comment   : Note: "sbjct" is synonymous with "hit"
 315
 316 =cut
 317
 318 =head2 overlap
 319
 320 See documentation in L<Bio::Search::Hit::HitI::overlap()|Bio::Search::Hit::HitI>
 321
 322 =cut
 323
 324 sub overlap {
 325     my $self = shift;
 326     $self->{'_overlap'} = shift if @_;
 327     return exists $self->{'_overlap'} ? $self->{'_overlap'} : 0;
 328 }
 329
 330
 331 =head2 n
 332
 333  Usage     : $hit_object->n();
 334  Purpose   : Gets the N number for the current hit.
 335            : This is the number of HSPs in the set which was ascribed
 336            : the lowest P-value (listed on the description line).
 337            : This number is not the same as the total number of HSPs.
 338            : To get the total number of HSPs, use num_hsps().
 339  Example   : $n = $hit_object->n();
 340  Returns   : Integer
 341  Argument  : n/a
 342  Throws    : Exception if HSPs have not been set.
 343  Comments  : Calling n() on such reports will result in a call to num_hsps().
 344            : The num_hsps() method will count the actual number of
 345            : HSPs in the alignment listing, which may exceed N in
 346            : some cases.
 347
 348 See Also   : L<num_hsps()|num_hsps>
 349
 350 =cut
 351
 352 sub n {
 353     my $self = shift;
 354     $self->{'_n'} = shift if @_;
 355     return exists $self->{'_n'} ? $self->{'_n'} : $self->num_hsps;
 356 }
 357
 358
 359 =head2 p
 360
 361  Usage     : $hit_object->p( [format] );
 362  Purpose   : Get the P-value for the best HSP
 363  Example   : $p =  $sbjct->p;
 364            : $p =  $sbjct->p('exp');  # get exponent only.
 365            : ($num, $exp) =  $sbjct->p('parts');  # split sci notation into parts
 366  Returns   : Float or scientific notation number (the raw P-value, DEFAULT).
 367            : Integer if format == 'exp' (the magnitude of the base 10 exponent).
 368            : 2-element list (float, int) if format == 'parts' and P-value
 369            :                is in scientific notation (See Comments).
 370  Argument  : format: string of 'raw' | 'exp' | 'parts'
 371            :    'raw' returns value given in report. Default. (1.2e-34)
 372            :    'exp' returns exponent value only (34)
 373            :    'parts' returns the decimal and exponent as a
 374            :            2-element list (1.2, -34) (See Comments).
 375  Throws    : Warns if no P-value is defined. Uses expect instead.
 376  Comments  : Using the 'parts' argument is not recommended since it will not
 377            : work as expected if the P-value is not in scientific notation.
 378            : That is, floats are not converted into sci notation before
 379            : splitting into parts.
 380
 381 See Also   : L<expect()|expect>, L<signif()|signif>, L<Bio::Search::SearchUtils::get_exponent()|Bio::Search::SearchUtils>
 382
 383 =cut
 384
 385 =head2 hsp
 386
 387  Usage     : $hit_object->hsp( [string] );
 388  Purpose   : Get a single HSPI object for the present HitI object.
 389  Example   : $hspObj  = $hit_object->hsp;  # same as 'best'
 390            : $hspObj  = $hit_object->hsp('best');
 391            : $hspObj  = $hit_object->hsp('worst');
 392  Returns   : Object reference for a Bio::Search::HSP::BlastHSP.pm object.
 393  Argument  : String (or no argument).
 394            :   No argument (default) = highest scoring HSP (same as 'best').
 395            :   'best' or 'first' = highest scoring HSP.
 396            :   'worst' or 'last' = lowest scoring HSP.
 397  Throws    : Exception if the HSPs have not been collected.
 398            : Exception if an unrecognized argument is used.
 399
 400 See Also   : L<hsps()|hsps>, L<num_hsps>()
 401
 402 =cut
 403
 404 sub hsp {
 405     my( $self, $option ) = @_;
 406     $option ||= 'best';
 407
 408     if (not ref $self->{'_hsps'}) {
 409         $self->throw("Can't get HSPs: data not collected.");
 410     }
 411
 412     my @hsps = $self->hsps;
 413
 414     return $hsps[0]      if $option =~ /best|first|1/i;
 415     return $hsps[$#hsps] if $option =~ /worst|last/i;
 416
 417     $self->throw("Can't get HSP for: $option\n" .
 418                  "Valid arguments: 'best', 'worst'");
 419 }
 420
 421 =head2 rank
 422
 423  Title   : rank
 424  Usage   : $obj->rank($newval)
 425  Function: Get/Set the rank of this Hit in the Query search list
 426            i.e. this is the Nth hit for a specific query
 427  Returns : value of rank
 428  Args    : newvalue (optional)
 429
 430
 431 =cut
 432
 433 sub rank {
 434     my $self = shift;
 435     return $self->{'_rank'} = shift if @_;
 436     return $self->{'_rank'} || 1;
 437 }
 438
 439 =head2 locus
 440
 441  Title   : locus
 442  Usage   : $locus = $hit->locus();
 443  Function: Retrieve the locus (if available) for the hit
 444  Returns : a scalar string (empty string if not set)
 445  Args    : none
 446
 447 =cut
 448
 449 sub locus {
 450     my ($self,$value) = @_;
 451     my $previous = $self->{'_locus'};
 452     if( defined $value || ! defined $previous ) {
 453       unless (defined $value) {
 454         if ($self->{'_name'} =~/(gb|emb|dbj|ref)\|(.*)\|(.*)/) {
 455                   $value = $previous = $3;
 456                 } else {
 457           $value = $previous = '';
 458         }
 459       }
 460           $self->{'_locus'} = $value;
 461     }
 462         return $previous;
 463 }
 464
 465 =head2 each_accession_number
 466
 467  Title   : each_accession_number
 468  Usage   : @each_accession_number = $hit->each_accession_number();
 469  Function: Get each accession number listed in the description of the hit.
 470            If there are no alternatives, then only the primary accession will
 471            be given
 472  Returns : list of all accession numbers in the description
 473  Args    : none
 474
 475 =cut
 476
 477 sub each_accession_number {
 478     my ($self,$value) = @_;
 479     my $desc = $self->{'_description'};
 480     #put primary accnum on the list
 481     my @accnums;
 482     push (@accnums,$self->{'_accession'});
 483     if( defined $desc )  {
 484       while ($desc =~ /(\b\S+\|\S*\|\S*\s?)/g) {
 485         my $id = $1;
 486         my ($acc, $version);
 487         if ($id =~ /(gb|emb|dbj|sp|pdb|bbs|ref|tp[gde])\|(.*)\|(.*)/) {
 488             ($acc, $version) = split /\./, $2;
 489         } elsif ($id =~ /(pir|prf|pat|gnl)\|(.*)\|(.*)/) {
 490             ($acc, $version) = split /\./, $3;
 491         } elsif( $id =~ /(gim|gi|bbm|bbs|lcl)\|(\d*)/) {
 492             $acc = $id;
 493         } elsif( $id =~ /(oth)\|(.*)\|(.*)\|(.*)/ ) { # discontinued...
 494             ($acc,$version) = ($2);
 495         } else {
 496                      #punt, not matching the db's at ftp://ftp.ncbi.nih.gov/blast/db/README
 497                      #Database Name                     Identifier Syntax
 498           #============================      ========================
 499           #GenBank                           gb|accession|locus
 500           #EMBL Data Library                 emb|accession|locus
 501           #DDBJ, DNA Database of Japan       dbj|accession|locus
 502           #NBRF PIR                          pir||entry
 503           #Protein Research Foundation       prf||name
 504           #SWISS-PROT                        sp|accession|entry name
 505           #Brookhaven Protein Data Bank      pdb|entry|chain
 506           #Patents                           pat|country|number
 507           #GenInfo Backbone Id               bbs|number
 508           #General database identifier           gnl|database|identifier
 509           #NCBI Reference Sequence           ref|accession|locus
 510           #Local Sequence identifier         lcl|identifier
 511               $acc=$id;
 512             }
 513             push(@accnums, $acc);
 514           }
 515     }
 516     return @accnums;
 517 }
 518
 519 =head2 tiled_hsps
 520
 521 See documentation in L<Bio::Search::SearchUtils::tile_hsps()|Bio::Search::SearchUtils>
 522
 523 =cut
 524
 525 =head2 query_length
 526
 527  Title   : query_length
 528  Usage   : $obj->query_length($newval)
 529  Function: Get/Set the query_length
 530  Returns : value of query_length (a scalar)
 531  Args    : on set, new value (a scalar or undef, optional)
 532
 533
 534 =cut
 535
 536 sub query_length {
 537     my $self = shift;
 538
 539     return $self->{'_query_length'} = shift if @_;
 540     return $self->{'_query_length'};
 541 }
 542
 543 =head2 ncbi_gi
 544
 545  Title   : ncbi_gi
 546  Usage   : $acc = $hit->ncbi_gi();
 547  Function: Retrieve the NCBI Unique ID (aka the GI #),
 548            if available, for the hit
 549  Returns : a scalar string (empty string if not set)
 550  Args    : none
 551
 552 =cut
 553
 554 sub ncbi_gi {
 555     my ($self,$value) = @_;
 556     my $previous = $self->{'_ncbi_gi'};
 557     if( defined $value || ! defined $previous ) {
 558         $value = $previous = '' unless defined $value;
 559         $self->{'_ncbi_gi'} = $value;
 560     }
 561         return $previous;
 562 }
 563
 564 =head1 ModelHit methods overridden in ModelHit
 565
 566 The following methods have been overridden due to their current reliance on
 567 sequence-based queries. They may be implemented in future versions of this class.
 568
 569 =head2 length_aln
 570
 571 =cut
 572
 573 sub length_aln {
 574     my $self = shift;
 575     $self->warn('$hit->length_aln not implemented for Model-based searches');
 576     return;
 577 }
 578
 579 =head2 gaps
 580
 581 =cut
 582
 583 sub gaps {
 584     my $self = shift;
 585     $self->warn('$hit->gaps not implemented for Model-based searches');
 586     return;
 587 }
 588
 589 =head2 matches
 590
 591 =cut
 592
 593 sub matches {
 594     my $self = shift;
 595     $self->warn('$hit->matches not implemented for Model-based searches');
 596     return;
 597 }
 598
 599 =head2 start
 600
 601 =cut
 602
 603 sub start {
 604     my $self = shift;
 605     $self->warn('$hit->start not implemented for Model-based searches');
 606     return;
 607 }
 608
 609
 610 =head2 end
 611
 612 =cut
 613
 614 sub end {
 615     my $self = shift;
 616     $self->warn('$hit->end not implemented for Model-based searches');
 617     return;
 618 }
 619
 620 =head2 range
 621
 622 =cut
 623
 624 sub range {
 625     my $self = shift;
 626     $self->warn('$hit->range not implemented for Model-based searches');
 627     return;
 628 }
 629
 630 =head2 frac_identical
 631
 632 =cut
 633
 634 sub frac_identical {
 635     my $self = shift;
 636     $self->warn('$hit->frac_identical not implemented for Model-based searches');
 637     return;
 638 }
 639
 640 =head2 frac_conserved
 641
 642 =cut
 643
 644 sub frac_conserved {
 645     my $self = shift;
 646     $self->warn('$hit->frac_conserved not implemented for Model-based searches');
 647     return;
 648 }
 649
 650 =head2 frac_aligned_query
 651
 652 =cut
 653
 654 sub frac_aligned_query {
 655     my $self = shift;
 656     $self->warn('$hit->frac_aligned_query not implemented for Model-based searches');
 657     return;
 658 }
 659
 660 =head2 frac_aligned_hit
 661
 662 =cut
 663
 664 sub frac_aligned_hit {
 665     my $self = shift;
 666     $self->warn('$hit->frac_aligned_hit not implemented for Model-based searches');
 667     return;
 668 }
 669
 670 =head2 num_unaligned_hit
 671
 672 =cut
 673
 674 *num_unaligned_sbjct = \&num_unaligned_hit;
 675
 676 sub num_unaligned_hit {
 677     my $self = shift;
 678     $self->warn('$hit->num_unaligned_hit/num_unaligned_sbjct not implemented for Model-based searches');
 679     return;
 680 }
 681
 682 =head2 num_unaligned_query
 683
 684 =cut
 685
 686 sub num_unaligned_query {
 687     my $self = shift;
 688     $self->warn('$hit->num_unaligned_query not implemented for Model-based searches');
 689     return;
 690 }
 691
 692 =head2 seq_inds
 693
 694 =cut
 695
 696 sub seq_inds {
 697     my $self = shift;
 698     $self->warn('$hit->seq_inds not implemented for Model-based searches');
 699     return;
 700 }
 701
 702 =head2 strand
 703
 704 =cut
 705
 706 sub strand {
 707     my $self = shift;
 708     $self->warn('$hit->strand not implemented for Model-based searches');
 709     return;
 710 }
 711
 712 =head2 frame
 713
 714 =cut
 715
 716 sub frame {
 717     my $self = shift;
 718     $self->warn('$hit->frame not implemented for Model-based searches');
 719     return;
 720 }
 721
 722 =head2 logical_length
 723
 724 =cut
 725
 726 sub logical_length {
 727     my $self = shift;
 728     $self->warn('$hit->logical_length not implemented for Model-based searches');
 729     return;
 730 }
 731
 732 1;